In the present era there is a rapid development of new methodology in the field of molecular biology. New Techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. The main purpose of this practical manual is to introduce the reader to a selection of the basic and important tools, preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of molecular biology. The techniques used in basic research biotechnology laboratories and diagnostic are covered in detail with purpose, principle, procedure, materials and reagents used. The starter research can gain hands on experiences from start to finish in the field of Molecular Biology.