This Biochemistry and Biotechnology Laboratory Manual has been written to provide a sourcebook of technical information on various experiments routinely performed in practical classes by Biology, Biotechnology, Pharmacy and Agriculture graduate and postgraduate students. The manual would be useful for students, teachers and researchers as a convenient and easy-to-understand resource for experimental details. Carbohydrates, Lipids, Proteins and Amino Acids, Enzymes, Nucleic Acids, and molecular techniques are included in the manual. Each chapter has been introduced briefly in order to provide the necessary information about the experiments covered. Important information about the experiment is provided as notes at the end of each experiment. There has been sufficient care taken to include every detail of the experiments, which can be used. These experiments will cover the practical syllabus of most Biochemistry and Biotechnology courses available at the undergraduate and post graduate levels.