Soil and fertilizer are the most important aspects of agricultural science. This laboratory manual is designed for research scientist, technicians and students working in the soil testing laboratories. It elaborately covers introductory soil science courses, and highlights the many aspects of soil science this will be an excellent aid in teaching and practical work in laboratory for the basic soil science course. This manual also describe about basic information of soil testing laboratories, soil sampling tools, soil sampling methods, processing of soil sampling, analytical methods and instrumental techniques. Seventeen exercises i.e. introduce soil important terms uses in a laboratory for chemical analysis, methods for collection of samples, principles of the instruments, determination of pH, electrical conductivity, organic carbon, nitrogen, phosphorous, potash, calcium-magnesium, gypsum requirement, lime requirement, sulphur, carbonates and bi-carbonates, micronutrients (Zn, Cu, Fe and Mn), boron and molybdenum have been included. These chapters will support undergraduate’s students to understand the practical aspects of soil properties and their management. in better way. This manual will also help in quick revision and recollect the vast soil science experiments.